Xyloglucan endo-transglycosylase (XET) functions in gelatinous layers of tension wood fibers in poplar--a glimpse into the mechanism of the balancing act of trees.
Identifieur interne : 003A31 ( Main/Exploration ); précédent : 003A30; suivant : 003A32Xyloglucan endo-transglycosylase (XET) functions in gelatinous layers of tension wood fibers in poplar--a glimpse into the mechanism of the balancing act of trees.
Auteurs : Nobuyuki Nishikubo [Suède] ; Tatsuya Awano ; Alicja Banasiak ; Veronica Bourquin ; Farid Ibatullin ; Ryo Funada ; Harry Brumer ; Tuula T. Teeri ; Takahisa Hayashi ; Björn Sundberg ; Ewa J. MellerowiczSource :
- Plant & cell physiology [ 0032-0781 ] ; 2007.
Descripteurs français
- KwdFr :
- MESH :
- cytologie : Populus.
- enzymologie : Bois, Populus.
- métabolisme : Glycosyltransferase, Protéines végétales.
- Métabolisme glucidique, Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Glycosyltransferases, Plant Proteins.
- cytology : Populus.
- enzymology : Populus, Wood.
- Carbohydrate Metabolism, Gene Expression Regulation, Plant.
Abstract
Tension wood is a specialized tissue of deciduous trees that functions in bending woody stems to optimize their position in space. Tension wood fibers that develop on one side of the stem have an increased potency to shrink compared with fibers on the opposite side, thus creating a bending moment. It is believed that the gelatinous (G) cell wall layer containing almost pure cellulose of tension wood fibers is pivotal to their shrinking. By analyzing saccharide composition and linkage in isolated G-layers of poplar, we found that they contain some matrix components in addition to cellulose, of which xyloglucan is the most abundant. Xyloglucan, xyloglucan endo-transglycosylase (XET) activity and xyloglucan endo-transglycosylase/hydrolase (XTH) gene products were detected in developing G-layers by labeling using CCRC-M1 monoclonal antibody, in situ incorporation of XXXG-SR and the polyclonal antibody to poplar PttXET16-34, respectively, indicating that xyloglucan is incorporated into the G-layer during its development. Moreover, several XTH transcripts were altered and were generally up-regulated in developing tension wood compared with normal wood. In mature G-fibers, XTH gene products were detected in the G-layers while the XET activity was evident in the adjacent S(2) wall layer. We propose that XET activity is essential for G-fiber shrinking by repairing xyloglucan cross-links between G- and S(2)-layers and thus maintaining their contact. Surprisingly, XTH gene products and XET activity persisted in mature G-fibers for several years, suggesting that the enzyme functions after cell death repairing the cross-links as they are being broken during the shrinking process.
DOI: 10.1093/pcp/pcm055
PubMed: 17504814
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Teeri</name></author><author><name sortKey="Hayashi, Takahisa" sort="Hayashi, Takahisa" uniqKey="Hayashi T" first="Takahisa" last="Hayashi">Takahisa Hayashi</name></author><author><name sortKey="Sundberg, Bjorn" sort="Sundberg, Bjorn" uniqKey="Sundberg B" first="Björn" last="Sundberg">Björn Sundberg</name></author><author><name sortKey="Mellerowicz, Ewa J" sort="Mellerowicz, Ewa J" uniqKey="Mellerowicz E" first="Ewa J" last="Mellerowicz">Ewa J. 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Teeri</name></author><author><name sortKey="Hayashi, Takahisa" sort="Hayashi, Takahisa" uniqKey="Hayashi T" first="Takahisa" last="Hayashi">Takahisa Hayashi</name></author><author><name sortKey="Sundberg, Bjorn" sort="Sundberg, Bjorn" uniqKey="Sundberg B" first="Björn" last="Sundberg">Björn Sundberg</name></author><author><name sortKey="Mellerowicz, Ewa J" sort="Mellerowicz, Ewa J" uniqKey="Mellerowicz E" first="Ewa J" last="Mellerowicz">Ewa J. Mellerowicz</name></author></analytic><series><title level="j">Plant & cell physiology</title><idno type="ISSN">0032-0781</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbohydrate Metabolism (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Glycosyltransferases (metabolism)</term><term>Plant Proteins (metabolism)</term><term>Populus (cytology)</term><term>Populus (enzymology)</term><term>Wood (enzymology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Bois (enzymologie)</term><term>Glycosyltransferase (métabolisme)</term><term>Métabolisme glucidique (MeSH)</term><term>Populus (cytologie)</term><term>Populus (enzymologie)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glycosyltransferases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Bois</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycosyltransferase</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Carbohydrate Metabolism</term><term>Gene Expression Regulation, Plant</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Métabolisme glucidique</term><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Tension wood is a specialized tissue of deciduous trees that functions in bending woody stems to optimize their position in space. Tension wood fibers that develop on one side of the stem have an increased potency to shrink compared with fibers on the opposite side, thus creating a bending moment. It is believed that the gelatinous (G) cell wall layer containing almost pure cellulose of tension wood fibers is pivotal to their shrinking. By analyzing saccharide composition and linkage in isolated G-layers of poplar, we found that they contain some matrix components in addition to cellulose, of which xyloglucan is the most abundant. Xyloglucan, xyloglucan endo-transglycosylase (XET) activity and xyloglucan endo-transglycosylase/hydrolase (XTH) gene products were detected in developing G-layers by labeling using CCRC-M1 monoclonal antibody, in situ incorporation of XXXG-SR and the polyclonal antibody to poplar PttXET16-34, respectively, indicating that xyloglucan is incorporated into the G-layer during its development. Moreover, several XTH transcripts were altered and were generally up-regulated in developing tension wood compared with normal wood. In mature G-fibers, XTH gene products were detected in the G-layers while the XET activity was evident in the adjacent S(2) wall layer. We propose that XET activity is essential for G-fiber shrinking by repairing xyloglucan cross-links between G- and S(2)-layers and thus maintaining their contact. Surprisingly, XTH gene products and XET activity persisted in mature G-fibers for several years, suggesting that the enzyme functions after cell death repairing the cross-links as they are being broken during the shrinking process.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17504814</PMID><DateCompleted><Year>2007</Year><Month>10</Month><Day>11</Day></DateCompleted><DateRevised><Year>2007</Year><Month>06</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0032-0781</ISSN><JournalIssue CitedMedium="Print"><Volume>48</Volume><Issue>6</Issue><PubDate><Year>2007</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Plant & cell physiology</Title><ISOAbbreviation>Plant Cell Physiol</ISOAbbreviation></Journal><ArticleTitle>Xyloglucan endo-transglycosylase (XET) functions in gelatinous layers of tension wood fibers in poplar--a glimpse into the mechanism of the balancing act of trees.</ArticleTitle><Pagination><MedlinePgn>843-55</MedlinePgn></Pagination><Abstract><AbstractText>Tension wood is a specialized tissue of deciduous trees that functions in bending woody stems to optimize their position in space. Tension wood fibers that develop on one side of the stem have an increased potency to shrink compared with fibers on the opposite side, thus creating a bending moment. It is believed that the gelatinous (G) cell wall layer containing almost pure cellulose of tension wood fibers is pivotal to their shrinking. By analyzing saccharide composition and linkage in isolated G-layers of poplar, we found that they contain some matrix components in addition to cellulose, of which xyloglucan is the most abundant. Xyloglucan, xyloglucan endo-transglycosylase (XET) activity and xyloglucan endo-transglycosylase/hydrolase (XTH) gene products were detected in developing G-layers by labeling using CCRC-M1 monoclonal antibody, in situ incorporation of XXXG-SR and the polyclonal antibody to poplar PttXET16-34, respectively, indicating that xyloglucan is incorporated into the G-layer during its development. Moreover, several XTH transcripts were altered and were generally up-regulated in developing tension wood compared with normal wood. In mature G-fibers, XTH gene products were detected in the G-layers while the XET activity was evident in the adjacent S(2) wall layer. We propose that XET activity is essential for G-fiber shrinking by repairing xyloglucan cross-links between G- and S(2)-layers and thus maintaining their contact. Surprisingly, XTH gene products and XET activity persisted in mature G-fibers for several years, suggesting that the enzyme functions after cell death repairing the cross-links as they are being broken during the shrinking process.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nishikubo</LastName><ForeName>Nobuyuki</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Forest Genetics and Plant Physiology, Umea Plant Science Center, SLU, S901 83 Umea, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Awano</LastName><ForeName>Tatsuya</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Banasiak</LastName><ForeName>Alicja</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Bourquin</LastName><ForeName>Veronica</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Ibatullin</LastName><ForeName>Farid</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Funada</LastName><ForeName>Ryo</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Brumer</LastName><ForeName>Harry</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Teeri</LastName><ForeName>Tuula T</ForeName><Initials>TT</Initials></Author><Author ValidYN="Y"><LastName>Hayashi</LastName><ForeName>Takahisa</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Sundberg</LastName><ForeName>Björn</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Mellerowicz</LastName><ForeName>Ewa J</ForeName><Initials>EJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>05</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>Plant Cell Physiol</MedlineTA><NlmUniqueID>9430925</NlmUniqueID><ISSNLinking>0032-0781</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.-</RegistryNumber><NameOfSubstance UI="D016695">Glycosyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.207</RegistryNumber><NameOfSubstance UI="C473049">xyloglucan - xyloglucosyltransferase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="N">Carbohydrate Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016695" MajorTopicYN="N">Glycosyltransferases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>5</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>10</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>5</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17504814</ArticleId><ArticleId IdType="pii">pcm055</ArticleId><ArticleId IdType="doi">10.1093/pcp/pcm055</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Awano, Tatsuya" sort="Awano, Tatsuya" uniqKey="Awano T" first="Tatsuya" last="Awano">Tatsuya Awano</name><name sortKey="Banasiak, Alicja" sort="Banasiak, Alicja" uniqKey="Banasiak A" first="Alicja" last="Banasiak">Alicja Banasiak</name><name sortKey="Bourquin, Veronica" sort="Bourquin, Veronica" uniqKey="Bourquin V" first="Veronica" last="Bourquin">Veronica Bourquin</name><name sortKey="Brumer, Harry" sort="Brumer, Harry" uniqKey="Brumer H" first="Harry" last="Brumer">Harry Brumer</name><name sortKey="Funada, Ryo" sort="Funada, Ryo" uniqKey="Funada R" first="Ryo" last="Funada">Ryo Funada</name><name sortKey="Hayashi, Takahisa" sort="Hayashi, Takahisa" uniqKey="Hayashi T" first="Takahisa" last="Hayashi">Takahisa Hayashi</name><name sortKey="Ibatullin, Farid" sort="Ibatullin, Farid" uniqKey="Ibatullin F" first="Farid" last="Ibatullin">Farid Ibatullin</name><name sortKey="Mellerowicz, Ewa J" sort="Mellerowicz, Ewa J" uniqKey="Mellerowicz E" first="Ewa J" last="Mellerowicz">Ewa J. Mellerowicz</name><name sortKey="Sundberg, Bjorn" sort="Sundberg, Bjorn" uniqKey="Sundberg B" first="Björn" last="Sundberg">Björn Sundberg</name><name sortKey="Teeri, Tuula T" sort="Teeri, Tuula T" uniqKey="Teeri T" first="Tuula T" last="Teeri">Tuula T. Teeri</name></noCountry><country name="Suède"><noRegion><name sortKey="Nishikubo, Nobuyuki" sort="Nishikubo, Nobuyuki" uniqKey="Nishikubo N" first="Nobuyuki" last="Nishikubo">Nobuyuki Nishikubo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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